Multiple Choice
Based on Figure 1, which of the following best describes where each enzyme functions?
21
views
6. Enzymes and Enzyme Kinetics
Enzymes
Struggling with Biochemistry?
Join thousands of students who trust us to help them ace their exams!Watch the first videoMultiple Choice
PCR (Polymerase Chain Reaction) requires the use of which enzyme to synthesize new strands of DNA?
A
Ligase
B
Taq DNA polymerase
C
Reverse transcriptase
D
RNA polymerase
Verified step by step guidance1
Understand the purpose of PCR: PCR is a technique used to amplify specific DNA sequences, creating millions of copies of a target DNA segment.
Identify the key enzyme required for DNA synthesis during PCR: The enzyme must be able to withstand the high temperatures used in the denaturation step of PCR.
Learn about Taq DNA polymerase: Taq DNA polymerase is a thermostable enzyme derived from the bacterium *Thermus aquaticus*. It is specifically used in PCR because it can function at high temperatures without denaturing.
Compare the other enzymes listed: Ligase is used to join DNA fragments, reverse transcriptase synthesizes DNA from RNA templates, and RNA polymerase synthesizes RNA from DNA templates. None of these are suitable for PCR amplification.
Conclude that Taq DNA polymerase is the correct enzyme for PCR, as it synthesizes new DNA strands during the extension step of the reaction.
Related Videos
Related Practice
