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Multiple Choice
Nucleic acids are assembled in the _____ direction.
A
5' to 3'
B
3' to 5'
C
C to N
D
N to C
Verified step by step guidance
1
Understand the structure of nucleic acids: Nucleic acids, such as DNA and RNA, are polymers made up of nucleotide monomers. Each nucleotide consists of a phosphate group, a sugar (deoxyribose in DNA or ribose in RNA), and a nitrogenous base.
Learn the directionality of nucleic acids: Nucleic acids have a specific orientation due to the structure of the sugar-phosphate backbone. The 5' end refers to the phosphate group attached to the 5th carbon of the sugar, while the 3' end refers to the hydroxyl group attached to the 3rd carbon of the sugar.
Understand the process of polymerization: During nucleic acid synthesis, nucleotides are added to the growing chain by forming phosphodiester bonds. This occurs between the 3' hydroxyl group of the last nucleotide in the chain and the phosphate group of the incoming nucleotide.
Recognize the correct direction of assembly: The polymerization process always proceeds in the 5' to 3' direction, meaning new nucleotides are added to the 3' end of the growing strand.
Eliminate incorrect options: The 3' to 5' direction is incorrect for nucleic acid assembly. 'C to N' and 'N to C' refer to protein synthesis, not nucleic acid synthesis. Therefore, the correct answer is 5' to 3'.