Table of contents

1. Introduction to Biochemistry4h 34m
2. Water3h 23m
3. Amino Acids8h 10m
4. Protein Structure10h 4m
5. Protein Techniques14h 5m
6. Enzymes and Enzyme Kinetics13h 38m
7. Enzyme Inhibition and Regulation 8h 42m
8. Protein Function 9h 41m
9. Carbohydrates7h 49m
10. Lipids5h 49m
11. Biological Membranes and Transport 6h 37m
12. Biosignaling9h 45m
Review 1: Nucleic Acids, Lipids, & Membranes2h 47m
Review 2: Biosignaling, Glycolysis, Gluconeogenesis, & PP-Pathway3h 12m
Review 3: Pyruvate & Fatty Acid Oxidation, Citric Acid Cycle, & Glycogen Metabolism2h 26m
Review 4: Amino Acid Oxidation, Oxidative Phosphorylation, & Photophosphorylation1h 48m

8. Protein Function

Hill Plot

Biochemistry

8. Protein Function

Hill Plot

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Multiple Choice

Which of the following situations would produce a Hill plot with a Hill coefficient ($n_H$) less than 1?

A protein exhibiting positive cooperativity in ligand binding

A protein exhibiting negative cooperativity in ligand binding

A protein with a single ligand binding site

A protein with non-cooperative (independent) ligand binding sites

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Verified step by step guidance

Step 1: Understand the concept of the Hill coefficient ($n_H$). The Hill coefficient is a measure of cooperativity in ligand binding. If $n_H > 1$, the protein exhibits positive cooperativity, meaning binding of one ligand increases the affinity for subsequent ligands. If $n_H = 1$, the binding is non-cooperative, meaning each ligand binds independently. If $n_H < 1$, the protein exhibits negative cooperativity, meaning binding of one ligand decreases the affinity for subsequent ligands.

Step 2: Analyze the situation where a protein exhibits positive cooperativity in ligand binding. Positive cooperativity results in a Hill coefficient greater than 1 ($n_H > 1$), so this situation would not produce a Hill plot with $n_H < 1$.

Step 3: Consider the situation where a protein exhibits negative cooperativity in ligand binding. Negative cooperativity results in a Hill coefficient less than 1 ($n_H < 1$), so this situation would produce a Hill plot with $n_H < 1$.

Step 4: Evaluate the situation where a protein has a single ligand binding site. A protein with only one binding site cannot exhibit cooperativity (positive or negative), as there are no additional binding sites to influence. This results in a Hill coefficient of exactly 1 ($n_H = 1$), indicating non-cooperative binding.

Step 5: Examine the situation where a protein has non-cooperative (independent) ligand binding sites. Non-cooperative binding means each ligand binds independently, resulting in a Hill coefficient of exactly 1 ($n_H = 1$). This situation would not produce a Hill plot with $n_H < 1$.