Table of contents

1. Introduction to Biochemistry4h 34m
2. Water3h 23m
3. Amino Acids8h 10m
4. Protein Structure10h 4m
5. Protein Techniques14h 5m
6. Enzymes and Enzyme Kinetics13h 38m
7. Enzyme Inhibition and Regulation 8h 42m
8. Protein Function 9h 41m
9. Carbohydrates7h 49m
10. Lipids5h 49m
11. Biological Membranes and Transport 6h 37m
12. Biosignaling9h 45m
Review 1: Nucleic Acids, Lipids, & Membranes2h 47m
Review 2: Biosignaling, Glycolysis, Gluconeogenesis, & PP-Pathway3h 12m
Review 3: Pyruvate & Fatty Acid Oxidation, Citric Acid Cycle, & Glycogen Metabolism2h 26m
Review 4: Amino Acid Oxidation, Oxidative Phosphorylation, & Photophosphorylation1h 48m

6. Enzymes and Enzyme Kinetics

Enzymes

Biochemistry

6. Enzymes and Enzyme Kinetics

Enzymes

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Multiple Choice

Which of the following is an enzyme that cleaves DNA at a specific sequence?

Helicase

RNA polymerase

DNA ligase

Restriction endonuclease

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Verified step by step guidance

Understand the role of enzymes in DNA manipulation: Restriction endonucleases are specialized enzymes that recognize specific nucleotide sequences in DNA and cleave the DNA at or near these sites. This is a key feature that distinguishes them from other enzymes involved in DNA processes.

Compare the functions of the given enzymes: Helicase unwinds the DNA double helix, RNA polymerase synthesizes RNA from a DNA template, and DNA ligase joins DNA fragments together. None of these enzymes cleave DNA at specific sequences.

Focus on the unique function of restriction endonucleases: These enzymes are used in molecular biology for cutting DNA at precise locations, which is essential for techniques like cloning and genetic engineering.

Learn the significance of sequence specificity: Restriction endonucleases recognize palindromic sequences (e.g., GAATTC) and cleave the DNA at these sites, creating either blunt or sticky ends depending on the enzyme.

Conclude that restriction endonucleases are the correct answer: Based on their ability to cleave DNA at specific sequences, restriction endonucleases are the enzyme described in the problem.