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Multiple Choice
What is the function of single-strand binding proteins (SSB) during DNA replication?
A
To join Okazaki fragments
B
To prevent the re-annealing of separated DNA strands
C
To unwind the DNA double helix
D
To synthesize new DNA strands
Verified step by step guidance
1
Understand the context of DNA replication: DNA replication is the process by which a cell duplicates its DNA, ensuring that each daughter cell receives an identical copy of the DNA.
Identify the role of single-strand binding proteins (SSB): During DNA replication, the double-stranded DNA is unwound to form two single strands. These single strands are prone to re-annealing or forming secondary structures.
Explore the function of SSB proteins: Single-strand binding proteins bind to the separated DNA strands to stabilize them and prevent them from re-annealing or forming secondary structures.
Clarify why SSB proteins do not join Okazaki fragments: Okazaki fragments are short DNA sequences synthesized on the lagging strand. The joining of these fragments is facilitated by DNA ligase, not SSB proteins.
Differentiate SSB proteins from other replication proteins: SSB proteins do not unwind the DNA double helix (a function of helicase) nor synthesize new DNA strands (a function of DNA polymerase). Their primary role is to maintain the stability of single-stranded DNA during replication.