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Multiple Choice
What is the primary function of single-strand binding proteins during DNA replication?
A
To synthesize new DNA strands
B
To unwind the DNA double helix
C
To prevent the re-annealing of separated DNA strands
D
To join Okazaki fragments
Verified step by step guidance
1
Understand the process of DNA replication: DNA replication is the process by which a cell duplicates its DNA, resulting in two identical copies. This process involves several enzymes and proteins that work together to ensure accurate replication.
Identify the role of single-strand binding proteins (SSBs): During DNA replication, the double-stranded DNA is unwound to form two single strands. These single strands are prone to re-annealing or forming secondary structures, which can interfere with replication.
Recognize the function of SSBs: Single-strand binding proteins bind to the separated single DNA strands to stabilize them and prevent them from re-annealing or forming secondary structures. This stabilization is crucial for the replication machinery to access the DNA template and synthesize new strands.
Differentiate from other proteins involved in replication: Unlike helicase, which unwinds the DNA double helix, or DNA polymerase, which synthesizes new DNA strands, SSBs specifically maintain the single-stranded state of DNA during replication.
Conclude the primary function: The primary function of single-strand binding proteins during DNA replication is to prevent the re-annealing of separated DNA strands, ensuring that the replication process proceeds smoothly.