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Multiple Choice
Which of the following reactions produces the oxygen ($\mathrm{O_2}$) released during photosynthesis?
A
The reduction of NADP$^+$ to NADPH
B
The conversion of glucose to pyruvate during glycolysis
C
The splitting of water molecules ($\mathrm{2H_2O \rightarrow 4H^+ + 4e^- + O_2}$) during the light-dependent reactions
D
The fixation of carbon dioxide ($\mathrm{CO_2}$) into glucose during the Calvin cycle
Verified step by step guidance
1
Step 1: Understand the context of photosynthesis. Photosynthesis consists of two main stages: the light-dependent reactions and the Calvin cycle. The light-dependent reactions occur in the thylakoid membranes of chloroplasts and involve the conversion of light energy into chemical energy, while the Calvin cycle occurs in the stroma and focuses on carbon fixation.
Step 2: Identify the process responsible for oxygen ($\mathrm{O_2}$) production. Oxygen is released during the light-dependent reactions of photosynthesis, specifically through the splitting of water molecules (a process called photolysis). This occurs when light energy excites chlorophyll molecules, leading to the extraction of electrons from water.
Step 3: Examine the chemical reaction for water splitting. The reaction is: $\mathrm{2H_2O \rightarrow 4H^+ + 4e^- + O_2}$. Here, water molecules are broken down into protons ($\mathrm{H^+}$), electrons ($\mathrm{e^-}$), and oxygen gas ($\mathrm{O_2}$). The oxygen gas is released as a byproduct.
Step 4: Compare the given options. The reduction of NADP$^+$ to NADPH, glycolysis, and carbon fixation during the Calvin cycle do not involve the production of oxygen. Only the splitting of water molecules during the light-dependent reactions produces oxygen.
Step 5: Conclude that the correct answer is the splitting of water molecules ($\mathrm{2H_2O \rightarrow 4H^+ + 4e^- + O_2}$) during the light-dependent reactions of photosynthesis.