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Multiple Choice
Which of the following is one way scientists are able to study operons in prokaryotic organisms?
A
By observing operon regulation in eukaryotic cells
B
By using reporter genes such as lacZ to monitor operon activity
C
By sequencing only the ribosomal RNA genes
D
By inhibiting all transcription with antibiotics
Verified step by step guidance
1
Understand the concept of an operon: An operon is a cluster of genes in prokaryotic organisms that are transcribed together as a single mRNA molecule. It includes regulatory sequences like the promoter and operator, which control the expression of the genes.
Learn about reporter genes: Reporter genes, such as lacZ, are genes that produce easily measurable products (e.g., β-galactosidase in the case of lacZ). These genes are often used as markers to study gene expression and regulation.
Recognize how reporter genes are used: Scientists can insert reporter genes into an operon to monitor its activity. For example, when the operon is active, the reporter gene will be transcribed and translated, producing a detectable product.
Eliminate incorrect options: Observing operon regulation in eukaryotic cells is not relevant because operons are primarily found in prokaryotes. Sequencing only ribosomal RNA genes does not provide information about operon activity. Inhibiting all transcription with antibiotics would prevent any study of gene regulation.
Conclude that the correct method is using reporter genes like lacZ to monitor operon activity, as this allows scientists to study the regulation and expression of operons in prokaryotic organisms.