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Multiple Choice
Why are yeast cells frequently used as hosts for cloning in DNA-based technology?
A
They are unable to recombine foreign DNA, ensuring genetic stability.
B
They have the fastest growth rate among all microorganisms.
C
They lack a nucleus, making DNA manipulation easier.
D
They are eukaryotic and can perform post-translational modifications similar to higher organisms.
Verified step by step guidance
1
Understand the context of the question: Yeast cells are commonly used in DNA-based technology due to their unique biological properties. The goal is to identify the correct reason why yeast cells are preferred as hosts for cloning.
Review the characteristics of yeast cells: Yeast cells are eukaryotic organisms, meaning they have a nucleus and organelles similar to higher organisms. This allows them to perform complex cellular processes, including post-translational modifications.
Clarify the concept of post-translational modifications: These are chemical changes made to proteins after they are synthesized. Examples include phosphorylation, glycosylation, and acetylation. These modifications are crucial for the proper functioning of proteins in higher organisms.
Compare yeast cells to other microorganisms: Unlike prokaryotic cells (e.g., bacteria), yeast cells can perform post-translational modifications, making them more suitable for cloning and expressing eukaryotic genes that require these modifications.
Conclude why yeast cells are ideal hosts: Yeast cells are eukaryotic and capable of performing post-translational modifications, which is essential for producing functional proteins similar to those in higher organisms. This makes them valuable tools in DNA-based technology.