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Multiple Choice
Which of the following does NOT increase the stability of eukaryotic mRNAs?
A
Removal of introns by splicing
B
Deadenylation of the poly(A) tail
C
Addition of a poly(A) tail
D
Addition of a 5' cap
Verified step by step guidance
1
Understand the role of mRNA stability in eukaryotic cells: Stability of mRNA is crucial for efficient translation and gene expression. Various modifications to mRNA can either increase or decrease its stability.
Review the function of intron removal by splicing: Splicing removes non-coding regions (introns) from pre-mRNA, leaving only coding regions (exons). This process is essential for producing mature mRNA, which is more stable and ready for translation.
Examine the addition of a poly(A) tail: The poly(A) tail is added to the 3' end of mRNA during processing. It protects mRNA from enzymatic degradation and aids in translation, thereby increasing stability.
Analyze the addition of a 5' cap: The 5' cap is added to the beginning of mRNA during processing. It prevents degradation by exonucleases and facilitates ribosome binding during translation, enhancing mRNA stability.
Understand deadenylation of the poly(A) tail: Deadenylation refers to the shortening or removal of the poly(A) tail. This process decreases mRNA stability, making it more susceptible to degradation. Therefore, deadenylation does NOT increase mRNA stability.