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Multiple Choice
Eukaryotic mRNA processing does NOT include which of the following?
A
Removal of introns by splicing
B
Replication of the mRNA molecule
C
Addition of a 5' cap
D
Polyadenylation at the 3' end
Verified step by step guidance
1
Understand the context of eukaryotic mRNA processing: Eukaryotic mRNA undergoes several modifications before it is translated into a protein. These modifications ensure the mRNA is stable, properly processed, and ready for translation.
Review the key steps of mRNA processing: These include (1) addition of a 5' cap, (2) removal of introns via splicing, and (3) addition of a poly-A tail at the 3' end (polyadenylation). These processes are essential for mRNA maturation.
Identify the incorrect option: Replication of the mRNA molecule is not part of mRNA processing. Replication refers to the duplication of DNA, not mRNA. mRNA is transcribed from DNA and processed, but it is not replicated.
Clarify why replication is not part of mRNA processing: mRNA is a single-stranded molecule that serves as a template for protein synthesis. It does not undergo replication because its role is temporary and functional, not hereditary like DNA.
Conclude that the correct answer is 'Replication of the mRNA molecule,' as it is not a step in eukaryotic mRNA processing.