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Multiple Choice
Which of the following is required for the termination of translation in eukaryotic cells?
A
Peptidyl transferase
B
Initiation factors
C
Release factors
D
Aminoacyl-tRNA synthetase
Verified step by step guidance
1
Understand the process of translation in eukaryotic cells: Translation is the process by which ribosomes synthesize proteins using mRNA as a template. It consists of three main stages: initiation, elongation, and termination.
Focus on the termination stage: Termination occurs when a stop codon (UAA, UAG, or UGA) is encountered on the mRNA. Stop codons do not code for any amino acid and signal the end of translation.
Learn the role of release factors: Release factors are proteins that recognize stop codons and bind to the ribosome. They facilitate the release of the newly synthesized polypeptide chain from the ribosome by promoting hydrolysis of the bond between the polypeptide and the tRNA in the P site.
Clarify why other options are incorrect: Peptidyl transferase is an enzyme involved in forming peptide bonds during elongation, not termination. Initiation factors are proteins that assist in the beginning of translation, and aminoacyl-tRNA synthetase is responsible for attaching amino acids to their corresponding tRNAs during translation preparation.
Conclude that release factors are essential for translation termination: Without release factors, the ribosome would not be able to recognize stop codons or release the completed polypeptide chain, halting the process of protein synthesis.