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Multiple Choice
Which of the following is NOT involved in the initiation of translation in bacteria?
A
30S ribosomal subunit
B
Initiator tRNA carrying formylmethionine (fMet)
C
RNA polymerase
D
Shine-Dalgarno sequence
Verified step by step guidance
1
Understand the process of translation initiation in bacteria: Translation is the process of synthesizing proteins from mRNA. In bacteria, initiation involves specific components and steps to assemble the ribosome and start translation.
Identify the key components involved in bacterial translation initiation: These include the 30S ribosomal subunit, the initiator tRNA carrying formylmethionine (fMet), and the Shine-Dalgarno sequence on the mRNA. These components work together to position the ribosome and tRNA correctly on the mRNA.
Clarify the role of the Shine-Dalgarno sequence: This sequence is a purine-rich region on the mRNA that binds to the complementary region on the 16S rRNA of the 30S ribosomal subunit, ensuring proper alignment of the ribosome with the start codon.
Explain why RNA polymerase is not involved: RNA polymerase is responsible for transcribing DNA into RNA during transcription, not translation. It plays no role in the initiation of translation in bacteria.
Conclude that RNA polymerase is the correct answer because it is not part of the translation initiation process, unlike the other listed components which are directly involved.