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Multiple Choice
In the rho-dependent mechanism of transcription termination, which of the following is required?
A
RNA polymerase
B
Poly-A tail
C
Rho protein
D
Sigma factor
Verified step by step guidance
1
Understand the concept of transcription termination: In prokaryotes, transcription termination can occur via two main mechanisms: rho-dependent and rho-independent. Rho-dependent termination requires the rho protein, while rho-independent relies on a specific sequence in the RNA.
Identify the role of the rho protein: The rho protein is a helicase that binds to the RNA and moves along it towards the RNA polymerase. It is essential for the rho-dependent termination mechanism.
Recognize the components involved in rho-dependent termination: The key components include the rho protein, RNA polymerase, and the RNA transcript. The rho protein interacts with the RNA transcript to facilitate termination.
Differentiate between the options provided: RNA polymerase is involved in transcription but not specifically in rho-dependent termination. The poly-A tail is related to eukaryotic mRNA processing, not prokaryotic transcription termination. The sigma factor is involved in the initiation of transcription, not termination.
Conclude that the rho protein is the required component for rho-dependent transcription termination, as it directly interacts with the RNA to terminate transcription.