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Multiple Choice
Which type of DNA library includes introns and promoters?
A
Plasmid library
B
cDNA library
C
Expression library
D
Genomic DNA library
Verified step by step guidance
1
Understand the concept of a DNA library: A DNA library is a collection of DNA fragments that are stored and propagated in host organisms, typically bacteria. These libraries are used for various purposes, including gene cloning and sequencing.
Differentiate between types of DNA libraries: There are several types of DNA libraries, including genomic DNA libraries, cDNA libraries, and expression libraries. Each type serves a different purpose and contains different types of DNA sequences.
Define a genomic DNA library: A genomic DNA library contains DNA fragments that represent the entire genome of an organism. This includes both coding regions (exons) and non-coding regions (introns, promoters, and other regulatory sequences).
Contrast with cDNA and expression libraries: A cDNA library is made from mRNA and represents only the expressed genes, excluding introns and promoters. An expression library is used to express proteins and typically contains cDNA sequences that can be transcribed and translated.
Conclude why a genomic DNA library includes introns and promoters: Since a genomic DNA library encompasses the entire genome, it naturally includes all types of sequences present in the DNA, such as introns and promoters, which are not found in cDNA or expression libraries.