Multiple Choice
What is the function of DNA ligase in the gene cloning process?
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18. Biotechnology
Introduction to DNA Cloning
Multiple Choice
What is recombinant DNA made of?
A
Restriction enzymes and target DNA.
B
Target DNA and DNA ligase.
C
DNA from two different sources.
D
A bacterial chromosome and a bacterial plasmid.
E
None are correct.
Verified step by step guidance1
Understand the concept of recombinant DNA: Recombinant DNA is a form of DNA that is created by combining DNA from two different sources. This process is fundamental in genetic engineering and biotechnology.
Learn about the role of restriction enzymes: Restriction enzymes are proteins that cut DNA at specific sequences. They are used to cut the DNA from different sources to create compatible ends for recombination.
Explore the function of DNA ligase: DNA ligase is an enzyme that joins DNA fragments together. After the DNA from different sources is cut by restriction enzymes, DNA ligase is used to seal the fragments together, forming recombinant DNA.
Identify the components involved in creating recombinant DNA: Typically, recombinant DNA involves a vector (such as a plasmid) and a piece of foreign DNA. The vector is used to carry the foreign DNA into a host cell.
Consider the applications of recombinant DNA: Recombinant DNA technology is used in various applications, including the production of insulin, growth hormones, and genetically modified organisms (GMOs).
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