18. Biotechnology
Introduction to DNA Cloning
- Multiple ChoiceWhat is the function of DNA ligase in the gene cloning process?353views
- Textbook Question
Which of the following is true of a codon?
a. It never codes for the same amino acid as another codon.
b. It can code for more than one amino acid.
c. It can be either in DNA or in RNA
d. It is the basic unit of protein structure
2689views - Multiple ChoiceWhich of the following is NOT a common step in molecular cloning using plasmids?10views
- Multiple Choice
Small accessory rings of DNA that replicate independently of an organism's genome are called _____.
1991views10rank - Multiple ChoiceWhich step of bacterial transformation occurs after a gene of interest has been isolated?403views
- Multiple ChoiceBacteria use restriction enzymes to __________.1297views
- Multiple Choice
In DNA technology, the term 'vector' can refer to:
2452views13rank - Multiple Choice
What is recombinant DNA made of?
2534views16rank - Multiple ChoiceGene cloning is crucial to any application involving one gene because __________.1152views
- Multiple ChoiceWhich of the following enzymes could seal a nick in one strand of a double-stranded DNA molecule by creating a sugar-phosphate bond between the adjacent, unjoined nucleotides?999views
- Multiple ChoiceThe efficiency of cloning, and the ability to generate healthy cloned animals, has been largely hampered by the difficulty of __________.1212views
- Multiple ChoiceIn genetic engineering, the highly active plasmid from Agrobacterium tumefaciens is used to __________.1079views
- Multiple ChoiceA molecular biologist used a retroviral vector to introduce a gene coding for a certain human enzyme into mouse cells. One cell line was isolated that was able to make the human enzyme, but it had lost the ability to express an endogenous, normally expressed gene in the process. What is the best explanation for these results?1095views
- Textbook Question
In DNA technology, the term vector can refer to
a. The enzyme that cuts DNA into restriction fragments.
b. The sticky end of a DNA fragment.
c. A SNP marker.
d. A plasmid used to transfer DNA into a living cell.
819views - Textbook Question
Which of the following would be considered a transgenic organism?
a. A bacterium that has received genes via conjugation
b. A human given a corrected human blood-clotting gene
c. A fern grown in cell culture from a single fern root cell
d. A rat with rabbit hemoglobin genes
1233views