A plaque assay is performed beginning with 1 mL of a solution containing bacteriophages. This solution is serially diluted three times by combining 0.1 mL of each sequential dilution with 9.9 mL of liquid medium. Then 0.1 mL of the final dilution is plated in the plaque assay and yields 17 plaques. What is the initial density of bacteriophages in the original 1 mL?
Table of contents
- 1. Introduction to Genetics42m
- 2. Mendel's Laws of Inheritance3h 37m
- 3. Extensions to Mendelian Inheritance2h 41m
- 4. Genetic Mapping and Linkage2h 28m
- 5. Genetics of Bacteria and Viruses1h 21m
- 6. Chromosomal Variation1h 48m
- 7. DNA and Chromosome Structure56m
- 8. DNA Replication1h 10m
- 9. Mitosis and Meiosis1h 34m
- 10. Transcription1h 0m
- 11. Translation58m
- 12. Gene Regulation in Prokaryotes1h 19m
- 13. Gene Regulation in Eukaryotes44m
- 14. Genetic Control of Development44m
- 15. Genomes and Genomics1h 50m
- 16. Transposable Elements47m
- 17. Mutation, Repair, and Recombination1h 6m
- 18. Molecular Genetic Tools19m
- 19. Cancer Genetics29m
- 20. Quantitative Genetics1h 26m
- 21. Population Genetics50m
- 22. Evolutionary Genetics29m
5. Genetics of Bacteria and Viruses
Bacteriophage Genetics
Multiple Choice
In bacteriophage genetics, which of the following types of bacteriophages may use RNA as its genome?
A
An RNA phage (e.g., a bacteriophage with a single-stranded RNA genome)
B
A bacteriophage that uses RNA only as a transient replication intermediate but has a DNA genome
C
A double-stranded DNA tailed phage (e.g., T4-like)
D
A bacteriophage that packages only host bacterial mRNA instead of its own genome
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Verified step by step guidance1
Step 1: Understand the types of genetic material that viruses, including bacteriophages, can have. Viruses may have DNA or RNA genomes, but not both as their main genetic material.
Step 2: Recognize that an RNA phage is a bacteriophage whose genome is composed of RNA, often single-stranded RNA (ssRNA). This means its hereditary information is stored directly in RNA.
Step 3: Differentiate this from bacteriophages that have DNA genomes but may use RNA transiently during replication. In these cases, RNA is an intermediate, not the genome itself.
Step 4: Identify that double-stranded DNA tailed phages (like T4) have DNA genomes, so they do not use RNA as their genetic material.
Step 5: Understand that bacteriophages packaging host bacterial mRNA instead of their own genome do not have RNA genomes; they are simply carrying host RNA, which is not their genetic material.
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