What experimental evidence confirms that we have introduced a useful gene into a transgenic organism and that it performs as we anticipate?

There are a variety of circumstances under which rapid results using multiple markers in PCR amplifications are highly desired, such as in forensics, pathogen analysis, or detection of genetically modified organisms. In multiplex PCR, multiple sets of primers are used, often with less success than when applied to PCR as individual sets. Numerous studies have been conducted to optimize procedures, but each has described the process as time consuming and often unsuccessful. Considering the information given in Problem 30, why should multiplex PCR be any different than single primer set PCR in terms of dependability and ease of optimization?

The purpose of polymerase chain reaction is to do what?

Which of the following lists the steps of genetic cloning in the proper order?

How can gene knockouts, transgenic animals, and gene editing techniques be used to explore gene function?

How has DNA-sequencing technology evolved in response to the emerging needs of genome scientists?

What steps make PCR a chain reaction that can produce millions of copies of a specific DNA molecule in a matter of hours without using host cells?

In a recombinant DNA cloning experiment, how can we determine whether DNA fragments of interest have been incorporated into plasmids and, once host cells are transformed, which cells contain recombinant DNA?

What purpose do the bla and lacZ genes serve in the plasmid vector pUC18?