Multiple Choice
In standard recombinant DNA-based gene cloning, what is typically the first step in the cloning workflow?
3
views
18. Molecular Genetic Tools
Genetic Cloning
Struggling with Genetics?
Join thousands of students who trust us to help them ace their exams!Watch the first videoMultiple Choice
The purpose of polymerase chain reaction is to do what?
A
Create RNA templates
B
Create fluorescent probes
C
Amplify a short DNA sequence
D
Isolate proteins
Verified step by step guidance1
Understand the purpose of Polymerase Chain Reaction (PCR): PCR is a technique used to amplify a specific segment of DNA, making millions of copies of a particular DNA sequence.
Recognize the components required for PCR: These include the DNA template that contains the target sequence, DNA polymerase enzyme, primers, nucleotides, and a buffer solution.
Learn the basic steps of PCR: The process involves three main steps - denaturation (heating the DNA to separate strands), annealing (cooling to allow primers to bind to the target sequence), and extension (DNA polymerase extends the primers to form a new DNA strand).
Identify the applications of PCR: PCR is used in various fields such as genetic research, forensic science, and medical diagnostics to amplify DNA for further analysis.
Clarify misconceptions: PCR is not used to create RNA templates, fluorescent probes, or isolate proteins. Its primary function is to amplify DNA sequences.
Related Videos
Related Practice
Genetic Cloning practice set
Problem sets built by lead tutorsExpert video explanations