Multiple Choice
In the context of genetic cloning and gene therapy, what is the primary purpose of a vector?
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18. Molecular Genetic Tools
Genetic Cloning
Multiple Choice
Which of the following lists the steps of genetic cloning in the proper order?
A
PCR → Restriction Enzyme Cutting → Ligate into a Vector → Placed into Organism
B
Restriction Enzyme Cutting → PCR → Ligate into a Vector → Placed into Organism
C
Ligate into a Vector → PCR → Restriction Enzyme Cutting → Placed into Organism
D
PCR → Ligate into a Vector → Restriction Enzyme Cutting → Placed into Organism
Verified step by step guidance1
Understand the purpose of each step in genetic cloning: PCR is used to amplify the DNA segment of interest, restriction enzyme cutting is used to create compatible ends for ligation, ligation into a vector is to insert the DNA into a carrier molecule, and placing into an organism is to introduce the vector into a host for replication.
Identify the first step: PCR (Polymerase Chain Reaction) is typically the initial step to amplify the DNA segment that you want to clone.
Determine the second step: Restriction enzyme cutting is used after PCR to cut the DNA and the vector at specific sites to create compatible ends for ligation.
Identify the third step: Ligate the DNA fragment into a vector, which involves joining the DNA fragment with the vector using DNA ligase to form a recombinant DNA molecule.
Determine the final step: Place the recombinant vector into an organism, such as bacteria, to allow for replication and expression of the cloned DNA.
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