The U.S. Department of Justice has established a database that catalogs PCR amplification products from short tandem repeats of the Y chromosome (Y-STRs) in humans. The database contains polymorphisms of five U.S. ethnic groups (African-Americans, European Americans, Hispanics, Native Americans, and Asian-Americans) as well as the worldwide population.
Given that STRs are repeats of varying lengths, for example (TCTG)₉₋₁₇ or (TAT)₆₋₁₄, explain how PCR could reveal differences (polymorphisms) among individuals. How could the Department of Justice make use of those differences?

Describe how having the Cas9 gene at a genomic locus unlinked to the guide RNA and target site locus in an engineered gene drive system could slow the propagation of the gene drive allele in a population into which a small number of individuals carrying both the gene drive allele and the Cas9 locus are released.

The U.S. Department of Justice has established a database that catalogs PCR amplification products from short tandem repeats of the Y chromosome (Y-STRs) in humans. The database contains polymorphisms of five U.S. ethnic groups (African-Americans, European Americans, Hispanics, Native Americans, and Asian-Americans) as well as the worldwide population.

For forensic applications, the probability of a 'match' for a crime scene DNA sample and a suspect's DNA often culminates in a guilty or innocent verdict. How is a 'match' determined, and what are the uses and limitations of such probabilities?

The U.S. Department of Justice has established a database that catalogs PCR amplification products from short tandem repeats of the Y chromosome (Y-STRs) in humans. The database contains polymorphisms of five U.S. ethnic groups (African-Americans, European Americans, Hispanics, Native Americans, and Asian-Americans) as well as the worldwide population.

What would be the value of knowing the ethnic population differences for Y-STR polymorphisms?

The U.S. Department of Justice has established a database that catalogs PCR amplification products from short tandem repeats of the Y chromosome (Y-STRs) in humans. The database contains polymorphisms of five U.S. ethnic groups (African-Americans, European Americans, Hispanics, Native Americans, and Asian-Americans) as well as the worldwide population.

Y-STRs from the nonrecombining region of the Y chromosome (NRY) have special relevance for forensic purposes. Why?

Would a gene drive system spread rapidly through a population in a species that tends to self-mate (e.g., Arabidopsis, C. elegans)? In a species in which the breeding cycle is slow (e.g., humans)?

There are a variety of circumstances under which rapid results using multiple markers in PCR amplifications are highly desired, such as in forensics, pathogen analysis, or detection of genetically modified organisms. In multiplex PCR, multiple sets of primers are used, often with less success than when applied to PCR as individual sets. Numerous studies have been conducted to optimize procedures, but each has described the process as time consuming and often unsuccessful. Considering the information given in Problem 30, why should multiplex PCR be any different than single primer set PCR in terms of dependability and ease of optimization?

The purpose of polymerase chain reaction is to do what?

Which of the following lists the steps of genetic cloning in the proper order?