For a eukaryotic gene whose transcription requires the activity of an enhancer sequence, explain how proteins bound at the enhancer interact with RNA pol II and transcription factors bound at the promoter.
Table of contents
- 1. Introduction to Genetics51m
- 2. Mendel's Laws of Inheritance3h 37m
- 3. Extensions to Mendelian Inheritance2h 41m
- 4. Genetic Mapping and Linkage2h 28m
- 5. Genetics of Bacteria and Viruses1h 21m
- 6. Chromosomal Variation1h 48m
- 7. DNA and Chromosome Structure56m
- 8. DNA Replication1h 10m
- 9. Mitosis and Meiosis1h 34m
- 10. Transcription1h 0m
- 11. Translation58m
- 12. Gene Regulation in Prokaryotes1h 19m
- 13. Gene Regulation in Eukaryotes44m
- 14. Genetic Control of Development44m
- 15. Genomes and Genomics1h 50m
- 16. Transposable Elements47m
- 17. Mutation, Repair, and Recombination1h 6m
- 18. Molecular Genetic Tools19m
- 19. Cancer Genetics29m
- 20. Quantitative Genetics1h 26m
- 21. Population Genetics50m
- 22. Evolutionary Genetics29m
10. Transcription
Transcription in Eukaryotes
Problem 15b
Textbook Question
The eukaryotic gene Gen-100 contains four introns labeled A to D. Imagine that Gen-100 has been isolated and its DNA has been denatured and mixed with polyadenylated mRNA from the gene.
Label the introns.

1
Understand the structure of eukaryotic genes: Eukaryotic genes consist of exons (coding regions) and introns (non-coding regions). Introns are removed during RNA splicing to produce mature mRNA.
Recognize that polyadenylated mRNA refers to mature mRNA, which has undergone splicing to remove introns and has a poly-A tail added at its 3' end.
Denature the DNA of the Gen-100 gene to separate its two strands, allowing hybridization with the mature mRNA.
Mix the denatured DNA with the mature mRNA. The exons in the mRNA will hybridize with their complementary sequences in the DNA, while the introns will form loops because they are absent in the mRNA.
Label the loops formed during hybridization as introns A, B, C, and D, corresponding to the non-coding regions of the Gen-100 gene that were spliced out during mRNA processing.

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Key Concepts
Here are the essential concepts you must grasp in order to answer the question correctly.
Introns and Exons
Introns are non-coding sequences within a gene that are transcribed into pre-mRNA but are removed during RNA splicing. Exons, on the other hand, are the coding sequences that remain in the mature mRNA and are translated into proteins. Understanding the distinction between introns and exons is crucial for analyzing gene structure and function.
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mRNA Processing
RNA Splicing
RNA splicing is the process by which introns are removed from the pre-mRNA transcript, and exons are joined together to form the final mRNA molecule. This process is essential for producing a functional mRNA that can be translated into a protein. Knowledge of splicing mechanisms helps in understanding how gene expression is regulated.
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Polyadenylation
Polyadenylation is the addition of a poly(A) tail to the 3' end of mRNA molecules, which enhances mRNA stability and facilitates its export from the nucleus to the cytoplasm. This modification is important for the maturation of mRNA and plays a role in the regulation of gene expression. Recognizing the significance of polyadenylation is key to understanding mRNA processing.
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