BackNeelaredoxin is a 15-kDa protein that is a gene product common in anaerobic bacteria. It has superoxide-scavenging activity, and it is constitutively expressed. In addition, its expression is not further induced during its exposure to O₂ or H₂O₂ [Silva, G. et al. (2001). J. Bacteriol. 183:4413 4420]. What do the terms constitutively expressed and induced mean in terms of neelaredoxin synthesis?
The creation of milk products such as cheeses and yogurts is dependent on the conversion by various anaerobic bacteria, including several Lactobacillus species, of lactose to glucose and galactose, ultimately producing lactic acid. These conversions are dependent on both permease and β-galactosidase as part of the lac operon. After selection for rapid fermentation for the production of yogurt, one Lactobacillus subspecies lost its ability to regulate lac operon expression [Lapierre, L., et al. (2002). J. Bacteriol. 184:928–935]. Would you consider it likely that in this subspecies the lac operon is on or off? What genetic events would likely contribute to the loss of regulation as described above?
In the lac operon, what are the likely effects on operon gene transcription of the mutations described in a–e?
Mutation of the CAP binding site of the lac promoter
In the lac operon, what are the likely effects on operon gene transcription of the mutations described in a–e?
Mutation of the lacI gene affecting the DNA-binding site of the protein
In the lac operon, what are the likely effects on operon gene transcription of the mutations described in a–e?
Mutation of the lacI gene affecting the allosteric site of the protein
In the lac operon, what are the likely effects on operon gene transcription of the mutations described in a–e?
Mutation of the repressor binding site on the operator sequence
In the lac operon, what are the likely effects on operon gene transcription of the mutations described in a–e?
Mutation of consensus sequence in the lac promoter
Identify which of the following lac operon haploid genotypes transcribe operon genes inducibly and which transcribe genes constitutively. Indicate whether the strain is lac⁺ (able to grow on lactose-only medium) or lac⁻ (cannot grow on lactose medium).
I⁺ P⁺ Oᶜ Z⁺ Y⁺
Identify which of the following lac operon haploid genotypes transcribe operon genes inducibly and which transcribe genes constitutively. Indicate whether the strain is lac⁺ (able to grow on lactose-only medium) or lac⁻ (cannot grow on lactose medium).
I⁺ P⁺ Oᶜ Z⁺ Y⁻
Identify which of the following lac operon haploid genotypes transcribe operon genes inducibly and which transcribe genes constitutively. Indicate whether the strain is lac⁺ (able to grow on lactose-only medium) or lac⁻ (cannot grow on lactose medium).
I⁺ P⁺ O⁺ Z⁻ Y⁺
Identify which of the following lac operon haploid genotypes transcribe operon genes inducibly and which transcribe genes constitutively. Indicate whether the strain is lac⁺ (able to grow on lactose-only medium) or lac⁻ (cannot grow on lactose medium).
I⁺ P⁻ O⁺ Z⁺ Y⁺
Identify which of the following lac operon haploid genotypes transcribe operon genes inducibly and which transcribe genes constitutively. Indicate whether the strain is lac⁺ (able to grow on lactose-only medium) or lac⁻ (cannot grow on lactose medium).
I⁻ P⁺ O⁺ Z⁺ Y⁺
Identify which of the following lac operon haploid genotypes transcribe operon genes inducibly and which transcribe genes constitutively. Indicate whether the strain is lac⁺ (able to grow on lactose-only medium) or lac⁻ (cannot grow on lactose medium).
I⁺ P⁺ Oᶜ Z⁻ Y⁺
Identify which of the following lac operon haploid genotypes transcribe operon genes inducibly and which transcribe genes constitutively. Indicate whether the strain is lac⁺ (able to grow on lactose-only medium) or lac⁻ (cannot grow on lactose medium).
I⁺ P⁺ O⁺ Z⁺ Y⁻
Complete the accompanying table, indicating whether functionally active -galactosidase and permease are produced in the presence and absence of lactose. Use '+' to indicate the presence of a functional enzyme and '−' to indicate its absence. Indicate whether the partial diploid strain is lac⁺ (able to grow on lactose-only medium) or lac⁻ (cannot grow on lactose medium).