18. Molecular Genetic Tools

In terms of molecular biology, what is a library?

If you had a sample of RNA to analyze, which of the following techniques would you most likely use?

What is CODIS? Describe the four most important features of genetic markers used in this system.

From GWAS, how do we know which genes are associated with a particular genetic disorder?

How does a positive ASO test for sickle-cell anemia determine that an individual is homozygous recessive for the mutation that causes sickle-cell anemia?

Compare and contrast the terms Paternity Index (PI) and Combined Paternity Index (CPI). How does each contribute to paternity determination?

What is the exclusion principle? How is it used in forensic genetic analysis and in paternity determination?

What is the statistical principle underlying genetic health risk assessment? Why are these assessments not predictive of disease occurrence?

You have constructed four different libraries: a genomic library made from DNA isolated from human brain tissue, a genomic library made from DNA isolated from human muscle tissue, a human brain cDNA library, and a human muscle cDNA library.

Which of these would have the greatest diversity of sequences?

You have constructed four different libraries: a genomic library made from DNA isolated from human brain tissue, a genomic library made from DNA isolated from human muscle tissue, a human brain cDNA library, and a human muscle cDNA library.

Would the sequences contained in each library be expected to overlap completely, partially, or not at all with the sequences present in each of the other libraries?

Using the genomic libraries, you wish to clone the human gene encoding myostatin, which is expressed only in muscle cells.

Assuming the human genome is 3x10⁹ bp and that the average insert size in the genomic libraries is 100 kb, how frequently will a clone representing myostatin be found in the genomic library made from muscle?

Explain the meaning of 'identity by descent' in the context of identifying genealogical relationship between individuals. In these analyses, why are segments of chromosomes (haplotypes) rather than individual STRs used to identify genetic relationships?

Using the genomic libraries, you wish to clone the human gene encoding myostatin, which is expressed only in muscle cells.

How frequently will a clone representing myostatin be found in the genomic library made from brain?

Using the genomic libraries, you wish to clone the human gene encoding myostatin, which is expressed only in muscle cells.

How frequently will a clone representing myostatin be found in the cDNA library made from muscle?

Using the genomic libraries, you wish to clone the human gene encoding myostatin, which is expressed only in muscle cells.

How frequently will a clone representing myostatin be found in the cDNA library made from brain?