17. Mutation, Repair, and Recombination

Two different mutations are identified in a haploid strain of yeast. The first prevents the synthesis of adenine by a nonsense mutation of the ade-1 gene. In this mutation, a base-pair substitution changes a tryptophan codon (UGG) to a stop codon (UGA). The second affects one of several duplicate tRNA genes. This base-pair substitution mutation changes the anticodon sequence of a tRNAᵀʳᵖ from

   3′−ACC−5′ to 3′−ACU−5′

Assuming there are no other mutations in the genome, will this double-mutant yeast strain be able to grow on minimal medium? If growth will occur, characterize the nature of growth relative to wild type.

A mutation occurred that changed the sequence 5' AAGCTTGC 3' to 5' AAGCTTTGC 3'. What is the name for this type of mutation?

Which of the following mutations changes one codon to a chemically different amino acid?

Which of the following point mutations changes a purine nucleotide to a pyrminidine nucleotide?

Changes in the codon reading frame can be caused from all but which of the following?

Identify the normal functions of the following genes whose mutations are associated with the development of cancer.

c-MYC(Burkitt lymphoma)

Identify the normal functions of the following genes whose mutations are associated with the development of cancer.

APC(familial adenomatous polyposis)

Identify the normal functions of the following genes whose mutations are associated with the development of cancer.

Which of these genes would you classify as a proto-oncogene and which as a tumor suppressor gene? Explain your categorization for each gene.

Identify the normal functions of the following genes whose mutations are associated with the development of cancer.

p53(Li–Fraumeni syndrome)