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Ch. 10 - Eukaryotic Chromosome Abnormalities and Molecular Organization
Sanders - Genetic Analysis: An Integrated Approach 3rd Edition
Sanders3rd EditionGenetic Analysis: An Integrated ApproachISBN: 9780135564172Not the one you use?Change textbook
Chapter 10, Problem 16d

The accompanying chromosome diagram represents a eukaryotic chromosome prepared with Giemsa stain. Indicate the heterochromatic and euchromatic regions of the chromosome, and label the chromosome's centromeric and telomeric regions.

Are you more likely to find the DNA sequence encoding the digestive enzyme amylase in a heterochromatic, euchromatic, centromeric, or telomeric region? Explain your reasoning.

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Examine the chromosome diagram prepared with Giemsa stain. Identify the darker-stained regions as heterochromatic regions and the lighter-stained regions as euchromatic regions. Heterochromatin is tightly packed DNA, while euchromatin is loosely packed and transcriptionally active.
Label the centromeric region of the chromosome. This is typically the constricted region of the chromosome where the kinetochore forms and spindle fibers attach during cell division.
Label the telomeric regions of the chromosome. These are the ends of the chromosome, which contain repetitive sequences that protect the chromosome from degradation.
Determine where the DNA sequence encoding the digestive enzyme amylase is likely to be found. Amylase is a protein-coding gene, so it is more likely to be located in a euchromatic region, as euchromatin is transcriptionally active and accessible for gene expression.
Explain the reasoning: Heterochromatic regions are transcriptionally inactive and primarily involved in structural roles, such as centromeres and telomeres. Since amylase is an actively expressed gene, it is unlikely to be found in heterochromatic, centromeric, or telomeric regions. Instead, it is most likely located in euchromatin, where genes are actively transcribed.

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Key Concepts

Here are the essential concepts you must grasp in order to answer the question correctly.

Euchromatin vs. Heterochromatin

Euchromatin is a less condensed form of chromatin that is actively involved in transcription, making it rich in genes and accessible for gene expression. In contrast, heterochromatin is tightly packed and generally transcriptionally inactive, often containing repetitive sequences and fewer genes. Understanding these differences is crucial for identifying where specific genes, like those encoding enzymes, are likely located.
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Centromere and Telomere

The centromere is the region of a chromosome that links sister chromatids and is essential for proper chromosome segregation during cell division. Telomeres are repetitive nucleotide sequences at the ends of chromosomes that protect them from degradation and prevent fusion with neighboring chromosomes. Both regions are typically not involved in gene encoding, which is important when considering where to find specific gene sequences.
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Gene Location and Function

The location of a gene within a chromosome can influence its expression and function. Genes that are actively expressed, such as those encoding enzymes like amylase, are usually found in euchromatic regions, where the chromatin is more open and accessible. This concept is vital for understanding why certain genes are located in specific chromosomal regions and how this affects their expression.
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Related Practice
Textbook Question

The accompanying chromosome diagram represents a eukaryotic chromosome prepared with Giemsa stain. Indicate the heterochromatic and euchromatic regions of the chromosome, and label the chromosome's centromeric and telomeric regions.

What term best describes the shape of this chromosome?

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Textbook Question

The accompanying chromosome diagram represents a eukaryotic chromosome prepared with Giemsa stain. Indicate the heterochromatic and euchromatic regions of the chromosome, and label the chromosome's centromeric and telomeric regions.

Do you expect the centromeric region to contain heterochromatin? Why or why not?

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Textbook Question

The accompanying chromosome diagram represents a eukaryotic chromosome prepared with Giemsa stain. Indicate the heterochromatic and euchromatic regions of the chromosome, and label the chromosome's centromeric and telomeric regions.

Why are expressed genes not found in the telomeric region of chromosomes?

556
views
Textbook Question

Histone protein H4 isolated from pea plants and cow thymus glands contains 102 amino acids in both cases. A total of 100 of the amino acids are identical between the two species. Give an evolutionary explanation for this strong amino acid sequence identity based on what you know about the functions of histones and nucleosomes.

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Textbook Question

A survey of organisms living deep in the ocean reveals two new species whose DNA is isolated for analysis. DNA samples from both species are treated to remove nonhistone proteins. Each DNA sample is then treated with DNase I that cuts DNA not protected by histone proteins but is unable to cut DNA bound by histone proteins. Following DNase I treatment, DNA samples are subjected to gel electrophoresis, and the gels are stained to visualize all DNA bands in the gel. The staining patterns of DNA bands from each species are shown in the figure. The number of base pairs in small DNA fragments is shown at the left of the gel. Interpret the gel results in terms of chromatin organization and the spacing of nucleosomes in the chromatin of each species.

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Textbook Question

In humans that are XX/XO mosaics, the phenotype is highly variable, ranging from females who have classic Turner syndrome symptoms to females who are essentially normal. Likewise, XY/XO mosaics have phenotypes that range from Turner syndrome females to essentially normal males. How can the wide range of phenotypes be explained for these sex-chromosome mosaics?

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