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Multiple Choice
Why are protons (H+) pumped across the inner mitochondrial membrane during the electron transport chain?
A
To create a proton gradient that drives ATP synthesis
B
To increase the pH inside the mitochondrial matrix
C
To transport electrons directly to oxygen
D
To directly produce ATP through substrate-level phosphorylation
Verified step by step guidance
1
Understand the role of the electron transport chain (ETC) in cellular respiration, which is to transfer electrons from electron donors to electron acceptors via redox reactions, and to couple this electron transfer with the transfer of protons (H+) across the inner mitochondrial membrane.
Recognize that the movement of protons across the inner mitochondrial membrane creates an electrochemical gradient, also known as the proton motive force. This gradient is essential for ATP synthesis.
Identify that the primary purpose of pumping protons across the membrane is to create a proton gradient. This gradient is used by ATP synthase, an enzyme that synthesizes ATP from ADP and inorganic phosphate.
Note that the increase in proton concentration in the intermembrane space (and decrease in the mitochondrial matrix) results in a lower pH in the intermembrane space compared to the matrix, but the main goal is not to increase pH inside the matrix.
Clarify that the protons do not directly transport electrons to oxygen nor directly produce ATP through substrate-level phosphorylation. Instead, they facilitate ATP production through oxidative phosphorylation, driven by the proton gradient.