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Multiple Choice
The tRNA shown in the figure has its 3′ end projecting beyond its 5′ end. Which of the following processes will occur at this 3′ end?
A
The amino acid binds covalently.
B
The excess nucleotides (ACCA) will be cleaved off at the ribosome.
C
The small and large subunits of the ribosome will attach to it.
D
These nucleotides represent the anti-codon.
Verified step by step guidance
1
Identify the structure of the tRNA molecule in the image. Notice that the tRNA has a cloverleaf structure with three loops and a stem, and the 3' end is longer than the 5' end.
Understand the function of the 3' end of the tRNA. The 3' end is where the amino acid attaches to the tRNA. This is crucial for the tRNA's role in protein synthesis.
Recognize that the sequence at the 3' end is typically CCA, which is conserved across all tRNA molecules. This sequence is essential for the attachment of the amino acid.
Explain that the process occurring at the 3' end is the covalent binding of an amino acid. This is facilitated by an enzyme called aminoacyl-tRNA synthetase, which attaches the correct amino acid to the tRNA based on its anticodon.
Clarify that the anticodon is located on a different part of the tRNA, specifically on one of the loops, and is responsible for base-pairing with the mRNA codon during translation. The 3' end is not involved in this base-pairing process.