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Multiple Choice
Which of the following processes generates a proton-motive force in mitochondria?
A
Glycolysis in the cytoplasm
B
ATP synthesis by ATP synthase
C
Electron transport through complexes I, III, and IV
D
Krebs cycle in the mitochondrial matrix
Verified step by step guidance
1
Understand the concept of proton-motive force: It is the force generated across the inner mitochondrial membrane due to the movement of protons (H⁺ ions) from the mitochondrial matrix to the intermembrane space.
Identify the role of the electron transport chain: The electron transport chain consists of complexes I, III, and IV, which are integral membrane proteins located in the inner mitochondrial membrane.
Recognize the function of these complexes: As electrons are transferred through these complexes, protons are pumped from the mitochondrial matrix into the intermembrane space, creating a gradient.
Explain the significance of the proton gradient: This gradient creates a potential energy difference across the membrane, known as the proton-motive force, which is essential for ATP synthesis.
Clarify why other processes do not generate this force: Glycolysis occurs in the cytoplasm and does not involve the mitochondrial membrane, ATP synthesis uses the proton-motive force but does not generate it, and the Krebs cycle occurs in the matrix without creating a proton gradient.