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Multiple Choice
Which of the following factors does NOT cause an enzyme to denature?
A
Presence of strong detergents
B
Extreme pH values
C
Mild changes in substrate concentration
D
High temperatures
Verified step by step guidance
1
Understand the concept of enzyme denaturation: Enzyme denaturation refers to the loss of the enzyme's three-dimensional structure, which is critical for its function. This can occur due to various environmental factors that disrupt the bonds maintaining the enzyme's structure.
Review the factors that cause denaturation: Factors such as extreme pH values, high temperatures, and strong detergents can disrupt hydrogen bonds, ionic bonds, and hydrophobic interactions within the enzyme, leading to denaturation.
Analyze the role of substrate concentration: Mild changes in substrate concentration do not affect the enzyme's structure directly. Instead, substrate concentration influences the rate of enzyme activity by affecting the availability of substrate for the enzyme to act upon, but it does not cause denaturation.
Compare mild substrate concentration changes with other factors: Unlike extreme pH values, high temperatures, or strong detergents, mild changes in substrate concentration do not disrupt the enzyme's structural integrity.
Conclude that mild changes in substrate concentration do NOT cause enzyme denaturation: This is because substrate concentration primarily affects enzyme kinetics rather than the enzyme's structural stability.