Table of contents

1. Introduction to Biochemistry4h 34m
2. Water3h 23m
3. Amino Acids8h 10m
4. Protein Structure10h 4m
5. Protein Techniques14h 5m
6. Enzymes and Enzyme Kinetics13h 38m
7. Enzyme Inhibition and Regulation 8h 42m
8. Protein Function 9h 41m
9. Carbohydrates7h 49m
10. Lipids5h 49m
11. Biological Membranes and Transport 6h 37m
12. Biosignaling9h 45m
Review 1: Nucleic Acids, Lipids, & Membranes2h 47m
Review 2: Biosignaling, Glycolysis, Gluconeogenesis, & PP-Pathway3h 12m
Review 3: Pyruvate & Fatty Acid Oxidation, Citric Acid Cycle, & Glycogen Metabolism2h 26m
Review 4: Amino Acid Oxidation, Oxidative Phosphorylation, & Photophosphorylation1h 48m

7. Enzyme Inhibition and Regulation

Enzyme Inhibition

Biochemistry

7. Enzyme Inhibition and Regulation

Enzyme Inhibition

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Multiple Choice

During the process of proofreading, what type of enzymatic activity does DNA polymerase utilize?

5' to 3' exonuclease activity

Endonuclease activity

Ligase activity

3' to 5' exonuclease activity

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Verified step by step guidance

Understand the concept of proofreading in DNA replication: DNA polymerase has the ability to correct errors during DNA synthesis by removing mismatched nucleotides.

Learn about exonuclease activity: Exonuclease enzymes remove nucleotides from the ends of a DNA strand. DNA polymerase uses exonuclease activity for proofreading.

Differentiate between 5' to 3' and 3' to 5' exonuclease activity: 5' to 3' exonuclease activity removes nucleotides from the 5' end, while 3' to 5' exonuclease activity removes nucleotides from the 3' end of the DNA strand.

Recognize that during proofreading, DNA polymerase uses 3' to 5' exonuclease activity to remove mismatched nucleotides from the growing DNA strand. This ensures high fidelity in DNA replication.

Exclude other enzymatic activities: Endonuclease activity cuts within the DNA strand, not at the ends, and ligase activity joins DNA fragments together. These are not involved in proofreading by DNA polymerase.