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Multiple Choice
In the context of the primary structure of a protein, what determines the order of amino acids in a protein?
A
The arrangement of multiple polypeptide subunits in a functional complex (quaternary structure)
B
The nucleotide sequence of the gene (DNA) as transcribed into mRNA codons
C
The pattern of hydrogen bonding between backbone atoms that forms -helices and -sheets
D
Post-translational modifications such as phosphorylation and glycosylation
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Verified step by step guidance
1
Understand that the primary structure of a protein refers to the linear sequence of amino acids in the polypeptide chain.
Recognize that this sequence is directly determined by the genetic information encoded in DNA, specifically the nucleotide sequence of a gene.
Recall that during gene expression, DNA is transcribed into messenger RNA (mRNA), which carries codons—triplets of nucleotides—that specify each amino acid.
Each mRNA codon corresponds to a specific amino acid, and the sequence of these codons dictates the order in which amino acids are assembled during translation.
Note that other options like quaternary structure, hydrogen bonding patterns, or post-translational modifications affect higher-level structures or protein function but do not determine the primary amino acid sequence.