Table of contents

1. Introduction to Biochemistry4h 34m
2. Water3h 23m
3. Amino Acids8h 10m
4. Protein Structure10h 4m
5. Protein Techniques14h 5m
6. Enzymes and Enzyme Kinetics13h 38m
7. Enzyme Inhibition and Regulation 8h 42m
8. Protein Function 9h 41m
9. Carbohydrates7h 49m
10. Lipids5h 49m
11. Biological Membranes and Transport 6h 37m
12. Biosignaling9h 45m
Review 1: Nucleic Acids, Lipids, & Membranes2h 47m
Review 2: Biosignaling, Glycolysis, Gluconeogenesis, & PP-Pathway3h 12m
Review 3: Pyruvate & Fatty Acid Oxidation, Citric Acid Cycle, & Glycogen Metabolism2h 26m
Review 4: Amino Acid Oxidation, Oxidative Phosphorylation, & Photophosphorylation1h 48m

3. Amino Acids

Amino Acid Configuration

Biochemistry

3. Amino Acids

Amino Acid Configuration

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Multiple Choice

In studies involving model tetrapeptides, to which atom is 7-amino-4-methylcoumarin (AMC) covalently attached?

The amino nitrogen of the N-terminal amino acid

The carboxyl oxygen of the C-terminal amino acid

The carboxyl carbon of the C-terminal amino acid

The alpha carbon of the N-terminal amino acid

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Verified step by step guidance

Understand the structure of a tetrapeptide: A tetrapeptide consists of four amino acids linked by peptide bonds. Each amino acid has an amino group (-NH2), a carboxyl group (-COOH), and a side chain (R group). The N-terminal amino acid is the one with a free amino group, and the C-terminal amino acid is the one with a free carboxyl group.

Learn about 7-amino-4-methylcoumarin (AMC): AMC is a fluorescent molecule often used in biochemical studies. It is typically covalently attached to peptides or proteins to enable detection or measurement of enzymatic activity.

Determine the attachment site: AMC is commonly attached to the carboxyl group of the C-terminal amino acid in peptides. This is because the carboxyl group (-COOH) can form a covalent bond with AMC through a chemical reaction, such as esterification or amidation.

Eliminate incorrect options: The amino nitrogen of the N-terminal amino acid is not typically involved in AMC attachment. Similarly, the alpha carbon of the N-terminal amino acid is not chemically reactive in this context. The carboxyl oxygen of the C-terminal amino acid is part of the carboxyl group, but the bond is formed with the carboxyl carbon.

Conclude the correct answer: The carboxyl carbon of the C-terminal amino acid is the atom to which AMC is covalently attached. This allows AMC to be linked to the peptide for experimental purposes.