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Multiple Choice
Which type of reaction is catalyzed by DNA ligase?
A
Hydrolysis of peptide bonds in proteins
B
Unwinding of the DNA double helix
C
Addition of methyl groups to DNA bases
D
Formation of phosphodiester bonds between DNA fragments
Verified step by step guidance
1
Understand the role of DNA ligase: DNA ligase is an enzyme that plays a critical role in DNA replication and repair by joining DNA fragments together.
Identify the type of bond formed: DNA ligase catalyzes the formation of phosphodiester bonds, which are covalent bonds between the phosphate group of one nucleotide and the hydroxyl group of another nucleotide.
Clarify the context of DNA fragments: During processes like DNA replication, Okazaki fragments are generated on the lagging strand. DNA ligase is responsible for sealing these fragments into a continuous strand by forming phosphodiester bonds.
Compare the given options: Hydrolysis of peptide bonds in proteins is catalyzed by proteases, unwinding of the DNA double helix is performed by helicases, and addition of methyl groups to DNA bases is carried out by methyltransferases. None of these are functions of DNA ligase.
Conclude the correct answer: DNA ligase specifically catalyzes the formation of phosphodiester bonds between DNA fragments, making it the correct answer.