Multiple Choice
In glycolysis, ATP molecules are produced directly by which process?
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Review 2: Biosignaling, Glycolysis, Gluconeogenesis, & PP-Pathway
Glycolysis 2
Multiple Choice
In glycolysis, how is ATP synthesized directly during the pathway?
A
By direct reduction of ADP to ATP using NADH as the phosphate donor
B
By substrate-level phosphorylation, where a phosphate group from a high-energy intermediate is transferred to ADP
C
By photophosphorylation using light energy absorbed by chlorophyll
D
By oxidative phosphorylation driven by the mitochondrial electron transport chain
Verified step by step guidance1
Understand that ATP synthesis in glycolysis occurs through a process called substrate-level phosphorylation, which is different from oxidative phosphorylation and photophosphorylation.
Identify that substrate-level phosphorylation involves the direct transfer of a phosphate group from a high-energy intermediate molecule to ADP, forming ATP without the involvement of the electron transport chain or light energy.
Recall the specific glycolytic intermediates involved in substrate-level phosphorylation, such as 1,3-bisphosphoglycerate and phosphoenolpyruvate, which donate their phosphate groups to ADP.
Recognize that NADH is not the phosphate donor in glycolysis; instead, NADH carries electrons and is involved in other processes like oxidative phosphorylation.
Summarize that the direct synthesis of ATP in glycolysis is by substrate-level phosphorylation, where a phosphate group from a high-energy intermediate is transferred directly to ADP, producing ATP.
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