Step 1: Understand the concept of ionizable groups. Ionizable groups in peptides are functional groups that can gain or lose protons depending on the pH. Common ionizable groups include the amino group (N-terminus), carboxyl group (C-terminus), and side chains of certain amino acids such as Aspartate, Glutamate, Lysine, Arginine, Histidine, Tyrosine, and Cysteine.