Multiple Choice
Which enzyme builds the new DNA strand by adding nucleotides during DNA replication?
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14. DNA Synthesis
DNA Polymerases
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Which of the following is not a property of DNA polymerase?
A
Can initiate DNA synthesis without a template
B
Requires a primer to initiate synthesis
C
Has proofreading activity to correct errors
D
Synthesizes DNA in the 5' to 3' direction
Verified step by step guidance1
Understand the role of DNA polymerase: DNA polymerase is an enzyme responsible for synthesizing new DNA strands by adding nucleotides to a pre-existing chain during DNA replication.
Identify the properties of DNA polymerase: DNA polymerase requires a template strand to guide the addition of complementary nucleotides, ensuring accurate DNA replication.
Recognize the need for a primer: DNA polymerase cannot initiate synthesis on its own; it requires a short RNA primer to provide a starting point for nucleotide addition.
Consider the proofreading activity: DNA polymerase has a proofreading function, which involves exonuclease activity that removes incorrectly paired nucleotides, ensuring high fidelity in DNA replication.
Determine the direction of synthesis: DNA polymerase synthesizes DNA in the 5' to 3' direction, meaning it adds nucleotides to the 3' end of the growing DNA strand.
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