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Multiple Choice
Which of the following correctly identifies the two main sources of hydrogen ions (protons) in the thylakoid space during photosynthesis?
A
Absorption of light by chlorophyll and ATP synthesis
B
Splitting of water (photolysis) and the transport of protons from the stroma via the electron transport chain
C
Oxidation of glucose and the Krebs cycle
D
Reduction of NADP$^+$ and the Calvin cycle
Verified step by step guidance
1
Understand the context: The question is about the sources of hydrogen ions (protons) in the thylakoid space during photosynthesis. This process occurs in the chloroplasts of plant cells, specifically during the light-dependent reactions of photosynthesis.
Step 1: Recall that the thylakoid space (lumen) is where the proton gradient is established during the light-dependent reactions. This gradient is essential for ATP synthesis via chemiosmosis.
Step 2: Identify the first source of protons: The splitting of water (photolysis). During photolysis, water molecules are split into oxygen (O₂), electrons, and hydrogen ions (H⁺). This reaction occurs in the thylakoid membrane and contributes protons directly to the thylakoid space.
Step 3: Identify the second source of protons: The transport of protons from the stroma into the thylakoid space via the electron transport chain (ETC). As electrons move through the ETC, energy is used to pump protons across the thylakoid membrane, increasing the proton concentration in the thylakoid space.
Step 4: Eliminate incorrect options: Absorption of light by chlorophyll and ATP synthesis do not directly contribute protons to the thylakoid space. Similarly, the oxidation of glucose, the Krebs cycle, and the Calvin cycle are not part of the light-dependent reactions and do not occur in the thylakoid space. Reduction of NADP⁺ occurs in the stroma, not the thylakoid space.