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Multiple Choice
DNA Polymerase III does not have which of the following activities?
A
Helicase activity
B
5' to 3' polymerase activity
C
5' to 3' exonuclease activity
D
3' to 5' exonuclease activity
Verified step by step guidance
1
Begin by understanding the role of DNA Polymerase III in DNA replication. It is primarily responsible for synthesizing new DNA strands by adding nucleotides in the 5' to 3' direction.
Next, consider the activities associated with DNA Polymerase III. It has 5' to 3' polymerase activity, which allows it to add nucleotides to the growing DNA strand.
DNA Polymerase III also possesses 3' to 5' exonuclease activity, which is crucial for proofreading and correcting errors during DNA synthesis.
Helicase activity is not a function of DNA Polymerase III. Helicases are separate enzymes that unwind the DNA double helix ahead of the replication fork.
Finally, note that DNA Polymerase III does not have 5' to 3' exonuclease activity. This activity is typically associated with other enzymes, such as DNA Polymerase I, which is involved in removing RNA primers and replacing them with DNA.