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Multiple Choice
Which of the following is a limitation of DNA polymerase?
A
DNA polymerase can initiate DNA synthesis without a primer.
B
DNA polymerase can synthesize RNA as well as DNA.
C
DNA polymerase can only synthesize DNA in the 5' to 3' direction.
D
DNA polymerase can repair mismatched bases during replication.
Verified step by step guidance
1
Understand the role of DNA polymerase: DNA polymerase is an enzyme responsible for synthesizing new DNA strands by adding nucleotides to a pre-existing chain during DNA replication.
Recognize the directionality of DNA synthesis: DNA polymerase can only add nucleotides to the 3' end of a growing DNA strand, meaning it synthesizes DNA in the 5' to 3' direction.
Identify the need for a primer: DNA polymerase cannot initiate DNA synthesis on its own; it requires a short RNA primer to provide a starting point for adding nucleotides.
Differentiate between DNA and RNA synthesis: DNA polymerase is specific to DNA synthesis and does not synthesize RNA. RNA synthesis is carried out by RNA polymerase.
Consider the proofreading ability: DNA polymerase has a proofreading function that allows it to remove and replace mismatched bases during DNA replication, ensuring high fidelity in DNA synthesis.