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Multiple Choice
In the process of DNA repair, what would be the consequence of omitting DNA ligase?
A
The DNA strands would remain fragmented and not properly joined.
B
The DNA strands would be over-replicated.
C
The DNA strands would be degraded by nucleases.
D
The DNA strands would be incorrectly replicated.
Verified step by step guidance
1
Understand the role of DNA ligase in DNA repair: DNA ligase is an enzyme that facilitates the joining of DNA strands together by catalyzing the formation of a phosphodiester bond. It is crucial in sealing nicks in the DNA backbone during DNA replication and repair.
Consider the process of DNA repair: During DNA repair, damaged or mismatched nucleotides are removed and replaced with the correct ones. After this replacement, the DNA backbone may have nicks or breaks that need to be sealed to restore the DNA's integrity.
Identify the consequence of omitting DNA ligase: Without DNA ligase, the nicks in the DNA backbone would not be sealed. This would result in fragmented DNA strands, as the phosphodiester bonds necessary to join the DNA fragments would not be formed.
Evaluate the options given: The correct consequence of omitting DNA ligase is that the DNA strands would remain fragmented and not properly joined. This is because the enzyme responsible for sealing the nicks is missing, preventing the completion of the repair process.
Conclude the analysis: The absence of DNA ligase would not lead to over-replication, degradation by nucleases, or incorrect replication directly. The primary issue is the failure to join DNA fragments, leading to incomplete and potentially unstable DNA molecules.