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Multiple Choice
Which enzyme is responsible for separating double-stranded DNA into single strands during DNA replication?
A
DNA polymerase
B
Ligase
C
Helicase
D
Primase
Verified step by step guidance
1
Understand the process of DNA replication: DNA replication is the process by which a double-stranded DNA molecule is copied to produce two identical DNA molecules. This process is essential for cell division.
Identify the role of enzymes in DNA replication: Several enzymes are involved in DNA replication, each with a specific function. Key enzymes include helicase, DNA polymerase, ligase, and primase.
Focus on the function of helicase: Helicase is the enzyme responsible for unwinding the double-stranded DNA. It breaks the hydrogen bonds between the nucleotide base pairs, separating the two strands to allow each strand to be copied.
Differentiate between the roles of other enzymes: DNA polymerase synthesizes the new DNA strand by adding nucleotides complementary to the template strand. Ligase joins Okazaki fragments on the lagging strand. Primase synthesizes RNA primers needed to start the DNA synthesis.
Conclude that helicase is the enzyme responsible for separating double-stranded DNA into single strands during DNA replication, as it unwinds the DNA helix.