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Ch. 12 DNA Technology and Genomics
Taylor - Campbell Biology: Concepts & Connections 10th Edition
Taylor, Simon, Dickey, Hogan10th EditionCampbell Biology: Concepts & ConnectionsISBN: 9780136538783Not the one you use?Change textbook
Chapter 12, Problem 7

Why does DNA profiling rely on comparing specific genetic markers rather than the entire genome?

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1
Understand that the human genome is very large, consisting of approximately 3 billion base pairs. Analyzing the entire genome for each individual would be time-consuming and costly.
Recognize that most of the human genome is identical among all people. DNA profiling focuses on specific regions that show high variability between individuals, which are more informative for identification purposes.
Learn about Short Tandem Repeats (STRs), which are the specific genetic markers often used in DNA profiling. STRs are sequences of DNA that repeat multiple times, and the number of repeats can vary greatly among individuals, making them useful for comparison.
Consider the practical application of DNA profiling in forensics, paternity tests, and genetic genealogy. By comparing STRs from a sample to a reference or between individuals, one can establish a genetic match or relationship with a high degree of certainty.
Acknowledge the efficiency of using specific markers: by targeting these variable regions, DNA profiling can be done quicker and with less genetic material, making it a practical tool for both legal and scientific purposes.

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Key Concepts

Here are the essential concepts you must grasp in order to answer the question correctly.

DNA Profiling

DNA profiling, also known as DNA fingerprinting, is a forensic technique used to identify individuals based on their unique genetic makeup. It focuses on specific regions of the DNA that vary greatly among individuals, allowing for accurate identification. This method is widely used in criminal investigations, paternity testing, and genetic research.
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Genetic Markers

Genetic markers are specific sequences in the DNA that can be used to identify individuals or species. These markers can be single nucleotide polymorphisms (SNPs), short tandem repeats (STRs), or other variations that are inherited and differ among individuals. By comparing these markers, scientists can determine genetic relationships and variations without needing to analyze the entire genome.
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Efficiency and Cost-effectiveness

Comparing specific genetic markers rather than the entire genome is more efficient and cost-effective. Analyzing the whole genome requires extensive resources and time, while focusing on a limited number of markers allows for quicker results and less computational power. This targeted approach also reduces the complexity of data interpretation, making it easier to draw conclusions.
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Related Practice
Textbook Question

A paleontologist has recovered a tiny bit of organic material from the 400-year-old preserved skin of an extinct dodo. She would like to compare DNA from the sample with DNA from living birds. Which of the following would be most useful for increasing the amount of DNA available for testing?

a. Restriction fragment analysis

b. Polymerase chain reaction

c. Molecular probe analysis

d. Electrophoresis

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Textbook Question

How many genes are there in a human sperm cell?

a. 23

b. 46

c. About 21,000

d. about 3 billion

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Textbook Question

When a typical restriction enzyme cuts a DNA molecule, the cuts are uneven, giving the DNA fragments single-stranded ends. These ends are useful in recombinant DNA work because

a. They enable a cell to recognize fragments produced by the enzyme.

b. They serve as starting points for DNA replication.

c. The fragments will bond to other fragments with complementary ends.

d. They enable researchers to use the fragments as molecular probes.

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Textbook Question
Recombinant DNA techniques are used to custom-build bacteria for two main purposes: to obtain multiple copies of certain genes and to obtain useful proteins produced by certain genes. Give an example of each of these applications in medicine and agriculture.
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Textbook Question
A biochemist hopes to find a gene in human cells that codes for an important blood-clotting protein. She knows that the nucleotide sequence of a small part of the blood-clotting gene is CTGGACTGACA. Briefly outline a possible method she might use to isolate the desired gene.
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Textbook Question

A biologist isolated a gene from a human cell, inserted it into a plasmid, and inserted the plasmid into a bacterium. The bacterium made a new protein, but it was nothing like the protein normally produced in a human cell. Why? (Explain your answer.)

a. The bacterium had undergone transformation.

b. The gene did not have sticky ends.

c. The human gene contained introns.

d. The gene was not synthesized from scratch.

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