Multiple Choice
During the DNA isolation process, which reagent is commonly used to stabilize DNA and prevent its degradation?
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17. Mutation, Repair, and Recombination
DNA Repair
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Join thousands of students who trust us to help them ace their exams!Watch the first videoMultiple Choice
Human DNA cut with restriction enzyme A can be joined to which of the following DNA fragments?
A
DNA fragments cut with any restriction enzyme
B
DNA fragments cut with the same restriction enzyme A
C
DNA fragments that have blunt ends only
D
DNA fragments that have no ends
Verified step by step guidance1
Understand that restriction enzymes cut DNA at specific sequences, producing fragments with characteristic ends, either 'sticky' (overhangs) or 'blunt' ends.
Recognize that for two DNA fragments to be joined (ligated) efficiently, their ends must be compatible; this usually means they are cut by the same restriction enzyme, producing complementary sticky ends or compatible blunt ends.
Recall that DNA fragments cut with different restriction enzymes generally have incompatible ends, preventing them from joining directly without additional modification.
Note that blunt ends can be joined to other blunt ends regardless of the enzyme used, but sticky ends require matching overhangs from the same enzyme.
Conclude that DNA fragments cut with restriction enzyme A can be joined only to DNA fragments cut with the same enzyme A, because they have compatible ends necessary for ligation.
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