Multiple Choice
In gene cloning projects, plasmids primarily function to:
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18. Molecular Genetic Tools
Genetic Cloning
Multiple Choice
In the context of genetic cloning, how does recombinant DNA technology differ from cloning?
A
Recombinant DNA technology relies exclusively on natural meiosis to shuffle alleles, whereas cloning relies on crossing over to create variation.
B
Recombinant DNA technology can only copy entire organisms, whereas cloning can only copy short DNA fragments in bacteria.
C
Recombinant DNA technology joins DNA fragments (often from different sources) to create a new DNA construct, whereas cloning produces genetically identical copies of a DNA segment, cell, or organism.
D
Recombinant DNA technology changes DNA only by random mutation, whereas cloning always edits DNA sequences using CRISPR.
Verified step by step guidance1
Understand the definitions: Recombinant DNA technology involves combining DNA fragments from different sources to create new DNA sequences, while cloning refers to producing genetically identical copies of DNA segments, cells, or entire organisms.
Recognize that recombinant DNA technology is a molecular technique used to manipulate and join DNA fragments, often to study genes or produce proteins, rather than copying whole organisms.
Note that cloning can occur at different biological levels: molecular cloning copies specific DNA fragments, cellular cloning produces identical cells, and organismal cloning creates whole organisms genetically identical to the original.
Distinguish that recombinant DNA technology does not rely on natural processes like meiosis or crossing over, but rather on laboratory methods such as restriction enzymes and ligases to join DNA pieces.
Clarify that cloning is not necessarily about editing DNA sequences (e.g., with CRISPR), but about replicating existing DNA exactly, whereas recombinant DNA technology is about creating new combinations of genetic material.
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