Multiple Choice
Which of the following polymerases is responsible for transcribing mRNA in eukaryotes?
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10. Transcription
Transcription in Eukaryotes
1:44 minutesProblem 1d
Textbook Question
How do we know that there is an association between disease susceptibility in humans and regulatory DNA sequences?
Verified step by step guidance1
Understand that regulatory DNA sequences control gene expression by influencing transcription levels, which can affect how genes function in different tissues or conditions.
Recognize that variations or mutations in these regulatory regions can alter gene expression patterns, potentially leading to differences in disease susceptibility among individuals.
Learn that researchers identify associations between disease susceptibility and regulatory DNA sequences by comparing genetic data from affected and unaffected individuals, looking for correlations between specific regulatory variants and disease presence.
Explore experimental methods such as genome-wide association studies (GWAS) that scan the genome for variants linked to diseases, including those in non-coding regulatory regions.
Consider functional assays where candidate regulatory sequences are tested in laboratory settings to observe their effect on gene expression, confirming their role in disease mechanisms.
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Key Concepts
Here are the essential concepts you must grasp in order to answer the question correctly.
Regulatory DNA Sequences
Regulatory DNA sequences are regions of the genome that control gene expression by influencing transcription. These include promoters, enhancers, silencers, and insulators, which interact with transcription factors to increase or decrease gene activity. Variations in these sequences can affect how genes are turned on or off, impacting cellular function and potentially contributing to disease.
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Genetic Association Studies
Genetic association studies identify correlations between specific genetic variants and traits or diseases in populations. By comparing DNA sequences of affected and unaffected individuals, researchers can pinpoint regulatory regions linked to disease susceptibility. These studies often use genome-wide association studies (GWAS) to find non-coding variants that influence gene regulation.
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Functional Validation of Regulatory Variants
Functional validation involves experimental methods to confirm that identified regulatory variants affect gene expression and contribute to disease. Techniques like reporter assays, CRISPR editing, and chromatin immunoprecipitation (ChIP) help demonstrate how changes in regulatory DNA alter transcription factor binding or chromatin state, linking genetic variation to biological effects and disease risk.
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