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Multiple Choice
Which of the following does NOT occur during eukaryotic pre-mRNA processing?
A
Addition of a poly(A) tail to the 3′ end of the transcript
B
Removal of introns by spliceosome-mediated splicing
C
Removal of exons to generate the mature mRNA
D
Addition of a 5′ cap to the nascent RNA transcript
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Verified step by step guidance
1
Understand the key processes involved in eukaryotic pre-mRNA processing, which include modifications that prepare the primary RNA transcript for translation.
Recall that the addition of a 5′ cap to the nascent RNA transcript is an essential early step that protects the RNA and assists in ribosome binding.
Recognize that the addition of a poly(A) tail to the 3′ end of the transcript is another important modification that enhances RNA stability and export from the nucleus.
Know that removal of introns by spliceosome-mediated splicing is the process that removes non-coding sequences (introns) from the pre-mRNA, joining the coding sequences (exons) together.
Identify that removal of exons to generate mature mRNA does NOT occur; instead, exons are retained and joined to form the final mRNA sequence.