Table of contents

1. A Review of General Chemistry5h 5m
2. Molecular Representations1h 14m
3. Acids and Bases2h 46m
4. Alkanes and Cycloalkanes4h 17m
5. Chirality3h 39m
6. Thermodynamics and Kinetics1h 22m
7. Substitution Reactions1h 48m
8. Elimination Reactions2h 30m
9. Alkenes and Alkynes2h 9m
10. Addition Reactions3h 19m
11. Radical Reactions1h 58m
12. Alcohols, Ethers, Epoxides and Thiols2h 42m
13. Alcohols and Carbonyl Compounds2h 17m
14. Synthetic Techniques1h 26m
15. Analytical Techniques:IR, NMR, Mass Spect7h 3m
16. Conjugated Systems6h 13m
17. Ultraviolet Spectroscopy51m
18. Aromaticity2h 34m
19. Reactions of Aromatics: EAS and Beyond5h 1m
20. Phenols55m
21. Aldehydes and Ketones: Nucleophilic Addition4h 56m
22. Carboxylic Acid Derivatives: NAS2h 51m
23. The Chemistry of Thioesters, Phophate Ester and Phosphate Anhydrides1h 10m
24. Enolate Chemistry: Reactions at the Alpha-Carbon1h 53m
25. Condensation Chemistry2h 9m
26. Amines1h 43m
27. Heterocycles2h 0m
28. Carbohydrates5h 53m
29. Amino Acids4h 20m
30. Peptides and Proteins2h 42m
31. Catalysis in Organic Reactions1h 30m
32. Lipids 2h 50m
33. The Organic Chemistry of Metabolic Pathways2h 52m
34. Nucleic Acids1h 32m
35. Transition Metals6h 14m
36. Synthetic Polymers1h 49m

36. Synthetic Polymers

Introduction to Polymers

Organic Chemistry

36. Synthetic Polymers

Introduction to Polymers

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Multiple Choice

Which of the following information is critical to the success of Polymerase Chain Reaction (PCR)?

The color of the DNA sample

The brand of the PCR machine

The sequence of the target DNA

The temperature of the laboratory

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Verified step by step guidance

Understand the purpose of Polymerase Chain Reaction (PCR): PCR is a technique used to amplify a specific segment of DNA, making millions of copies of a particular DNA sequence.

Identify the critical components required for PCR: These include the DNA template containing the target sequence, primers that are complementary to the target sequence, DNA polymerase enzyme, nucleotides, and a buffer solution.

Recognize the importance of the DNA sequence: The sequence of the target DNA is crucial because it determines the design of the primers. Primers must be complementary to the sequences flanking the target region to initiate DNA synthesis.

Consider the role of temperature in PCR: PCR involves repeated cycles of heating and cooling to denature the DNA, anneal the primers, and extend the DNA strands. The precise temperature settings are critical for the success of the reaction.

Evaluate the non-critical factors: The color of the DNA sample and the brand of the PCR machine do not affect the success of PCR. The key is the accurate design of primers based on the target DNA sequence and the correct thermal cycling conditions.