7. Enzyme Inhibition and Regulation

Use the data in the chart below to determine the answer to the following:

A) Rank the enzymes in order of their binding affinity to their substrate (strongest affinity → weakest affinity).

a) A → B → C.

b) C → A → B.

c) C → B → A.

d) B → A → C.

B) Rank the enzymes in order of their binding affinity to the inhibitor (strongest affinity → weakest affinity).

a) A → B → C.

b) B → C → A.

c) C → B → A.

d) B → A → C.

C) Which enzyme would you expect the inhibitor to affect the most? Why?

a) Enzyme A.

b) Enzyme B.

c) Enzyme C.

Calculate the Michaelis constant (K_m) and the inhibition constant (K _I) given the following information:

[E] = 20 mM. [S] = 15 mM. [ES] = 5 mM. [I] = 8 mM. [EI] = 2 mM.